Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human delta protocadherin 1 full ectodomains on membranes, tomogram 2

ABSTRACT:

SUBMITTER: Lawrence Shapiro

PROVIDER: EMPIAR-10368 | biostudies-other |

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Publications

Family-wide Structural and Biophysical Analysis of Binding Interactions among Non-clustered δ-Protocadherins.

Harrison Oliver J OJ Brasch Julia J Katsamba Phinikoula S PS Ahlsen Goran G Noble Alex J AJ Dan Hanbin H Sampogna Rosemary V RV Potter Clinton S CS Carragher Bridget B Honig Barry B Shapiro Lawrence L

Cell reports 20200201 8

Non-clustered δ1- and δ2-protocadherins, close relatives of clustered protocadherins, function in cell adhesion and motility and play essential roles in neural patterning. To understand the molecular interactions underlying these functions, we used solution biophysics to characterize binding of δ1- and δ2-protocadherins, determined crystal structures of ectodomain complexes from each family, and assessed ectodomain assembly in reconstituted intermembrane junctions by cryoelectron tomography (cry ...[more]

PMID: 32101743

Similar Datasets

Human delta protocadherin 1 full ectodomains on membranes, tomogram 1

Project description: Not available

| EMPIAR-10367 | biostudies-other

Human delta protocadherin 1 full ectodomains on membranes, tomogram 3

Project description: Not available

| EMPIAR-10369 | biostudies-other

Human delta protocadherin 10 full ectodomains on membranes, tomogram 1

Project description: Not available

| EMPIAR-10370 | biostudies-other

Human delta protocadherin 10 full ectodomains on membranes, tomogram 2

Project description: Not available

| EMPIAR-10371 | biostudies-other

Human delta protocadherin 10 full ectodomains on membranes, tomogram 3

Project description: Not available

| EMPIAR-10372 | biostudies-other

Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.

Project description:Recent studies revealed a striking difference in the genomic organization of classic cadherin genes and one family of "nonclassic cadherin" genes designated protocadherins. Specifically, the DNA sequences encoding the ectodomain repeats of classic cadherins are interrupted by multiple introns. By contrast, all of the encoded ectodomains of each member of the protocadherin gene clusters are present in one large exon. To determine whether large ectodomain exons are a general feature of protocadherin genes we have investigated the genomic organization of several additional human protocadherin genes by using DNA sequence information in GenBank. These genes include protocadherin 12 (Pcdh12), an ortholog of the mouse vascular endothelial cadherin-2 gene; hFmi1 and hFmi2, homologs of the Drosophila planar cell polarity gene, flamingo; hFat2, a homolog of the Drosophila tumor suppressor gene fat; and the Drosophila DN-cadherin and DE-cadherin genes. Each of these genes was found to be a member of the protocadherin subfamily, based on amino acid sequence comparisons of their ectodomains. Remarkably, all of these protocadherin genes share a common feature: most of the genomic DNA sequences encoding their ectodomains are not interrupted by an intron. We conclude that the presence of unusually large exons is a characteristic feature of protocadherin genes.

| S-EPMC16203 | biostudies-literature

Tuning of delta-protocadherin adhesion through combinatorial diversity.

Project description:The delta-protocadherins (?-Pcdhs) play key roles in neural development, and expression studies suggest they are expressed in combination within neurons. The extent of this combinatorial diversity, and how these combinations influence cell adhesion, is poorly understood. We show that individual mouse olfactory sensory neurons express 0-7 ?-Pcdhs. Despite this apparent combinatorial complexity, K562 cell aggregation assays revealed simple principles that mediate tuning of ?-Pcdh adhesion. Cells can vary the number of ?-Pcdhs expressed, the level of surface expression, and which ?-Pcdhs are expressed, as different members possess distinct apparent adhesive affinities. These principles contrast with those identified previously for the clustered protocadherins (cPcdhs), where the particular combination of cPcdhs expressed does not appear to be a critical factor. Despite these differences, we show ?-Pcdhs can modify cPcdh adhesion. Our studies show how intra- and interfamily interactions can greatly amplify the impact of this small subfamily on neuronal function.

| S-EPMC6326727 | biostudies-literature

Delta Protocadherin 10 is Regulated by Activity in the Mouse Main Olfactory System.

Project description:Olfactory sensory neurons (OSNs) are thought to use activity-dependent and independent mechanisms to regulate the expression of axon guidance genes. However, defining the molecular mechanisms that underlie activity-dependent OSN guidance has remained elusive. Only a handful of genes have been identified whose expression is regulated by activity. Interestingly, all of these genes have been shown to play a role in OSN axon guidance, underscoring the importance of identifying other genes regulated by activity. Furthermore, studies suggest that more than one downstream mechanism regulates target gene expression. Thus, both the number of genes regulated by activity and how many total mechanisms control this expression are not well understood. Here we identify delta protocadherin 10 (pcdh10) as a gene regulated by activity. Delta protocadherins are members of the cadherin superfamily, and pcdh10 is known to be important for axon guidance during development. We show pcdh10 is expressed in the nasal epithelium and olfactory bulb in patterns consistent with providing guidance information to OSNs. We use naris occlusion, genetic manipulations, and pharmacological assays to show pcdh10 can be regulated by activity, consistent with activation via the cyclic nucleotide-gated channel. Transgenic analysis confirms a potential role for pcdh10 in OSN axon guidance.

| S-EPMC3159872 | biostudies-literature

CryoET of cis-mutated mouse protocadherin gamma B6 on membranes

Project description: Not available

| EMPIAR-10236 | biostudies-other

CryoET of mouse protocadherin gamma B6 on membranes (without energy filter)

Project description: Not available

| EMPIAR-10237 | biostudies-other