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Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging - single particle cryoEM - workflow - on TMV

ABSTRACT:

SUBMITTER: Mikhail Kudryashev

PROVIDER: EMPIAR-10393 | biostudies-other |

REPOSITORIES: biostudies-other

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Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM.

Sanchez Ricardo M RM Zhang Yingyi Y Chen Wenbo W Dietrich Lea L Kudryashev Mikhail M

Nature communications 20200724 1

Cryo-electron tomography combined with subtomogram averaging (StA) has yielded high-resolution structures of macromolecules in their native context. However, high-resolution StA is not commonplace due to beam-induced sample drift, images with poor signal-to-noise ratios (SNR), challenges in CTF correction, and limited particle number. Here we address these issues by collecting tilt series with a higher electron dose at the zero-degree tilt. Particles of interest are then located within reconstru ...[more]

PMID: 32709843

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