Dataset Information
Jack bean urease imaged at 39kX nominal magnification
Ontology highlight
ABSTRACT:
SUBMITTER: J. Christopher Fromme
PROVIDER: EMPIAR-10549 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Experimental evaluation of super-resolution imaging and magnification choice in single-particle cryo-EM.
Journal of structural biology: X 20210313
The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit by increasing sampling frequency, yet there are few reports of reconstructions that do so. Here we directly examine the contribution of super-resolution information, obtained with the K3 direct electron det ...[more]