Ontology highlight
ABSTRACT:
SUBMITTER: J. Christopher Fromme
PROVIDER: EMPIAR-10656 | biostudies-other |
REPOSITORIES: biostudies-other
Feathers J Ryan JR Spoth Katherine A KA Fromme J Christopher JC
Journal of structural biology: X 20210313
The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit by increasing sampling frequency, yet there are few reports of reconstructions that do so. Here we directly examine the contribution of super-resolution information, obtained with the K3 direct electron det ...[more]