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Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana

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SUBMITTER: David Mark Lawson 

PROVIDER: EMPIAR-10560 | biostudies-other |

REPOSITORIES: biostudies-other

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Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.

Castells-Graells Roger R   Ribeiro Jonas R S JRS   Domitrovic Tatiana T   Hesketh Emma L EL   Scarff Charlotte A CA   Johnson John E JE   Ranson Neil A NA   Lawson David M DM   Lomonossoff George P GP  

Communications biology 20210524 1

Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these remain poorly understood. As a tool for studying virus maturation, we transiently expressed the capsid protein of the insect virus Nudaurelia capensis omega virus (NωV) in Nicotiana benthamiana and were able to purify both immature procapsids and mature capsids from infiltrated leaves by varying the expression time. Cryo-EM analysis of the plant-produced procapsi  ...[more]

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