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Single-particle cryo-EM micrographs of a chromatosome containing human linker histone H1.4

ABSTRACT:

SUBMITTER: Bing-Rui Zhou

PROVIDER: EMPIAR-10574 | biostudies-other |

REPOSITORIES: biostudies-other

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Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.

Zhou Bing-Rui BR Feng Hanqiao H Kale Seyit S Fox Tara T Khant Htet H de Val Natalia N Ghirlando Rodolfo R Panchenko Anna R AR Bai Yawen Y

Molecular cell 20201124 1

The repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1. There are 11 human H1 isoforms with diverse cellular functions, but how they interact with the nucleosome remains elusive. Here, we determined the cryoelectron microscopy (cryo-EM) structures of chromatosomes containing 197 bp DNA and three different human H1 isoforms, respectively. The globular domains of all three H1 isoforms bound to the nucleosome dyad. However, the flanking/l ...[more]

PMID: 33238161

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