Ribosome sample deposited using the chameleon (54 ms delay)
Ontology highlight
ABSTRACT:
SUBMITTER: David Paul Klebl
PROVIDER: EMPIAR-10603 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales.
Klebl David P DP Gravett Molly S C MSC Kontziampasis Dimitrios D Wright David J DJ Bon Robin S RS Monteiro Diana C F DCF Trebbin Martin M Sobott Frank F White Howard D HD Darrow Michele C MC Thompson Rebecca F RF Muench Stephen P SP
Structure (London, England : 1993) 20200818 11
A host of new technologies are under development to improve the quality and reproducibility of cryoelectron microscopy (cryoEM) grid preparation. Here we have systematically investigated the preparation of three macromolecular complexes using three different vitrification devices (Vitrobot, chameleon, and a time-resolved cryoEM device) on various timescales, including grids made within 6 ms (the fastest reported to date), to interrogate particle behavior at the air-water interface for different ...[more]
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