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Cryo-EM dataset of the substrate-engaged human 26S proteasome

ABSTRACT:

SUBMITTER: Youdong Jack Mao

PROVIDER: EMPIAR-10669 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome.

Dong Yuanchen Y Zhang Shuwen S Wu Zhaolong Z Li Xuemei X Wang Wei Li WL Zhu Yanan Y Stoilova-McPhie Svetla S Lu Ying Y Finley Daniel D Mao Youdong Y

Nature 20181112 7737

The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. Here we present cryo-electron microscopy structures of the substrate-engaged human proteasome in seven conformational states at 2.8-3.6 Å resolution, captured during breakdown of a polyubiquitylated protein. These structures illuminate a spatiotemporal continuum of dynamic substrate-proteasome interactions from ubiquitin recognition to substrate transloc ...[more]

PMID: 30479383

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