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Materials for step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo

ABSTRACT:

SUBMITTER: Daniel Castaño-Díez

PROVIDER: EMPIAR-10702 | biostudies-other |

REPOSITORIES: biostudies-other

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Step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo.

Scaramuzza Stefano S Castaño-Díez Daniel D

PLoS biology 20210826 8

Subtomogram averaging (STA) is a powerful image processing technique in electron tomography used to determine the 3D structure of macromolecular complexes in their native environments. It is a fast growing technique with increasing importance in structural biology. The computational aspect of STA is very complex and depends on a large number of variables. We noticed a lack of detailed guides for STA processing. Also, current publications in this field often lack a documentation that is practical ...[more]

PMID: 34437529

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