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VHUT-cryo-FIB, a method to fabricate frozen-hydrated lamella of tissue specimen for in situ cryo-electron tomography

ABSTRACT:

SUBMITTER: Sun F

PROVIDER: EMPIAR-10713 | biostudies-other |

REPOSITORIES: biostudies-other

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VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography.

Zhang Jianguo J Zhang Danyang D Sun Lei L Ji Gang G Huang Xiaojun X Niu Tongxin T Xu Jiashu J Ma Chengying C Zhu Yun Y Gao Ning N Xu Wei W Sun Fei F

Journal of structural biology 20210624 3

Cryo-electron tomography (cryo-ET) provides a promising approach to study intact structures of macromolecules in situ, but the efficient preparation of high-quality cryosections represents a bottleneck. Although cryo-focused ion beam (cryo-FIB) milling has emerged for large and flat cryo-lamella preparation, its application to tissue specimens remains challenging. Here, we report an integrated workflow, VHUT-cryo-FIB, for efficiently preparing frozen hydrated tissue lamella that can be readily u ...[more]

PMID: 34174447

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