Cryo electron microscopy of single particles of the Haliangium ochraceum Encapsulin:encapsulated ferritin encapsulin nano compartment
Ontology highlight
ABSTRACT:
SUBMITTER: Jon Marles-Wright
PROVIDER: EMPIAR-10716 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment.
Ross Jennifer J McIver Zak Z Lambert Thomas T Piergentili Cecilia C Bird Jasmine Emma JE Gallagher Kelly J KJ Cruickshank Faye L FL James Patrick P Zarazúa-Arvizu Efrain E Horsfall Louise E LE Waldron Kevin J KJ Wilson Marcus D MD Mackay C Logan CL Baslé Arnaud A Clarke David J DJ Marles-Wright Jon J
Science advances 20220126 4
Encapsulins are protein nanocompartments that house various cargo enzymes, including a family of decameric ferritin-like proteins. Here, we study a recombinant <i>Haliangium ochraceum</i> encapsulin:encapsulated ferritin complex using cryo-electron microscopy and hydrogen/deuterium exchange mass spectrometry to gain insight into the structural relationship between the encapsulin shell and its protein cargo. An asymmetric single-particle reconstruction reveals four encapsulated ferritin decamers ...[more]
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