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Single particle Data of diazaborine bound Drg1

ABSTRACT:

SUBMITTER: David Haselbach

PROVIDER: EMPIAR-10717 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.

Prattes Michael M Grishkovskaya Irina I Hodirnau Victor-Valentin VV Rössler Ingrid I Klein Isabella I Hetzmannseder Christina C Zisser Gertrude G Gruber Christian C CC Gruber Karl K Haselbach David D Bergler Helmut H

Nature communications 20210609 1

The hexameric AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis and initiates cytoplasmic maturation of the large ribosomal subunit by releasing the shuttling maturation factor Rlp24. Drg1 monomers contain two AAA-domains (D1 and D2) that act in a concerted manner. Rlp24 release is inhibited by the drug diazaborine which blocks ATP hydrolysis in D2. The mode of inhibition was unknown. Here we show the first cryo-EM structure of Drg1 revealing the inhibitory mechanism. Diazaborine ...[more]

PMID: 34108481

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| S-EPMC5651765 | biostudies-literature

Single Particle CryoEM of inactive SSTR2 bound to Nb6