Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM
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ABSTRACT:
SUBMITTER: Nikolaus Grigorieff
PROVIDER: EMPIAR-10731 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Locating macromolecular assemblies in cells by 2D template matching with cisTEM.
Lucas Bronwyn A BA Himes Benjamin A BA Xue Liang L Grant Timothy T Mahamid Julia J Grigorieff Nikolaus N
eLife 20210611
For a more complete understanding of molecular mechanisms, it is important to study macromolecules and their assemblies in the broader context of the cell. This context can be visualized at nanometer resolution in three dimensions (3D) using electron cryo-tomography, which requires tilt series to be recorded and computationally aligned, currently limiting throughput. Additionally, the high-resolution signal preserved in the raw tomograms is currently limited by a number of technical difficulties ...[more]
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