Dataset Information
E. coli 70S-RNAP expressome complex in collided state (without NusG, 34nt intervening mRNA) [5874 multi-frame micrographs in TIFF format]
Ontology highlight
ABSTRACT:
SUBMITTER: Albert Weixlbaumer
PROVIDER: EMPIAR-10845 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis of transcription-translation coupling and collision in bacteria.
Science (New York, N.Y.) 20200820 6509
Prokaryotic messenger RNAs (mRNAs) are translated as they are transcribed. The lead ribosome potentially contacts RNA polymerase (RNAP) and forms a supramolecular complex known as the expressome. The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures representing uncoupled, coupled, and collided expressome states determined by cryo-electron microscopy. A bridge between the ribosome and RNAP can be for ...[more]