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Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy

ABSTRACT:

SUBMITTER: Cornelius Thilo Gross

PROVIDER: EMPIAR-10883 | biostudies-other |

REPOSITORIES: biostudies-other

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Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy.

Ayuso-Jimeno Irene P IP Ronchi Paolo P Wang Tianzi T Gallori Catherine E CE Gross Cornelius T CT

Scientific reports 20220617 1

Enzymes that facilitate the local deposition of electron dense reaction products have been widely used as labels in electron microscopy (EM) for the identification of synaptic contacts in neural tissue. Peroxidases, in particular, can efficiently metabolize 3,3'-diaminobenzidine tetrahydrochloride hydrate (DAB) to produce precipitates with high contrast under EM following heavy metal staining, and can be genetically encoded to facilitate the labeling of specific cell-types or organelles. Neverth ...[more]

PMID: 35715545

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