Dataset Information
CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to an inhibitor corallopyronin and us-fork DNA
Ontology highlight
ABSTRACT:
SUBMITTER: Elizabeth A Campbell
PROVIDER: EMPIAR-10897 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature 20190109 7739
A key regulated step of transcription is promoter melting by RNA polymerase (RNAP) to form the open promoter complex<sup>1-3</sup>. To generate the open complex, the conserved catalytic core of the RNAP combines with initiation factors to locate promoter DNA, unwind 12-14 base pairs of the DNA duplex and load the template-strand DNA into the RNAP active site. Formation of the open complex is a multi-step process during which transient intermediates of unknown structure are formed<sup>4-6</sup>. ...[more]