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Cryo-EM multiframe micrographs of human Pol kappa complexed with DNA and monoubiquitylated PCNA

ABSTRACT:

SUBMITTER: Claudia Lancey

PROVIDER: EMPIAR-10934 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA.

Lancey Claudia C Tehseen Muhammad M Bakshi Souvika S Percival Matthew M Takahashi Masateru M Sobhy Mohamed A MA Raducanu Vlad S VS Blair Kerry K Muskett Frederick W FW Ragan Timothy J TJ Crehuet Ramon R Hamdan Samir M SM De Biasio Alfredo A

Nature communications 20211019 1

Y-family DNA polymerase κ (Pol κ) can replicate damaged DNA templates to rescue stalled replication forks. Access of Pol κ to DNA damage sites is facilitated by its interaction with the processivity clamp PCNA and is regulated by PCNA mono-ubiquitylation. Here, we present cryo-EM reconstructions of human Pol κ bound to DNA, an incoming nucleotide, and wild type or mono-ubiquitylated PCNA (Ub-PCNA). In both reconstructions, the internal PIP-box adjacent to the Pol κ Polymerase-Associated Domain ( ...[more]

PMID: 34667155

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