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Cryo-EM structure of the folate-specific ECF transporter complex in DDM micelles (200 kV)

ABSTRACT:

SUBMITTER: Cristina Paulino

PROVIDER: EMPIAR-10941 | biostudies-other |

REPOSITORIES: biostudies-other

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Insights into the bilayer-mediated toppling mechanism of a folate-specific ECF transporter by cryo-EM.

Thangaratnarajah Chancievan C Rheinberger Jan J Paulino Cristina C Slotboom Dirk J DJ

Proceedings of the National Academy of Sciences of the United States of America 20210801 34

Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes (∼115 kDa) that consist of a membrane-embedded, substrate-binding protein (S component) and a tripartite ATP-hydrolyzing module (ECF module). They import micronutrients into bacterial cells and have been proposed to use a highly unusual transport mechanism, in which the substrate is dragged across the membrane by a toppling motion of the S component. However, it remains unclear how the lipid bilayer c ...[more]

PMID: 34408021

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