Dataset Information
Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to AMP-PNP (200 kV)
Ontology highlight
ABSTRACT:
SUBMITTER: Cristina Paulino
PROVIDER: EMPIAR-11308 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Expulsion mechanism of the substrate-translocating subunit in ECF transporters.
Nature communications 20230725 1
Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF module) Previous data indicate that the S-component topples within the membrane to alternately expose the binding site to either side of the membrane. In many ECF transporters, the substrate-free S-component can be expelled from the ECF module. Here we study this enigmatic expulsion step by cryogeni ...[more]