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Cryo-EM structure of human RNA polymerase I Open Complex and in complex with RRN3

ABSTRACT:

SUBMITTER: Christoph W Müller

PROVIDER: EMPIAR-10966 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structures of human RNA polymerase I.

Misiaszek Agata D AD Girbig Mathias M Grötsch Helga H Baudin Florence F Murciano Brice B Lafita Aleix A Müller Christoph W CW

Nature structural & molecular biology 20211209 12

RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å resolution. In the exit tunnel, we observe a double-stranded RNA helix that may support Pol I processivity. Our structure confirms that human Pol I consists of 13 subunits with only one subunit forming the Pol I stalk. Additionally, the structure of h ...[more]

PMID: 34887565

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