Ontology highlight
ABSTRACT:
SUBMITTER: Fabian Eisenstein
PROVIDER: EMPIAR-10985 | biostudies-other |
REPOSITORIES: biostudies-other
Eisenstein Fabian F Yanagisawa Haruaki H Kashihara Hiroka H Kikkawa Masahide M Tsukita Sachiko S Danev Radostin R
Nature methods 20221201 1
In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in recent years as a powerful technique for structural studies of macromolecular complexes in their native cellular environment. However, the possibilities for recording tomographic tilt series in a high-throughput manner are limited, in part by the lamella-shaped samples. Here we utilize a geometrical sample model and optical image shift to record tens of tilt series in parallel, thereby saving time and gaining ...[more]