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Parallel cryo electron tomography (PACE-tomo) of 70S ribosomes


ABSTRACT:

SUBMITTER: Fabian Eisenstein 

PROVIDER: EMPIAR-10985 | biostudies-other |

REPOSITORIES: biostudies-other

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Parallel cryo electron tomography on in situ lamellae.

Eisenstein Fabian F   Yanagisawa Haruaki H   Kashihara Hiroka H   Kikkawa Masahide M   Tsukita Sachiko S   Danev Radostin R  

Nature methods 20221201 1


In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in recent years as a powerful technique for structural studies of macromolecular complexes in their native cellular environment. However, the possibilities for recording tomographic tilt series in a high-throughput manner are limited, in part by the lamella-shaped samples. Here we utilize a geometrical sample model and optical image shift to record tens of tilt series in parallel, thereby saving time and gaining  ...[more]

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