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Parallel cryo electron tomography (PACE-tomo) of 80S ribosomes in situ

ABSTRACT:

SUBMITTER: Fabian Eisenstein

PROVIDER: EMPIAR-10987 | biostudies-other |

REPOSITORIES: biostudies-other

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Parallel cryo electron tomography on in situ lamellae.

Eisenstein Fabian F Yanagisawa Haruaki H Kashihara Hiroka H Kikkawa Masahide M Tsukita Sachiko S Danev Radostin R

Nature methods 20221201 1

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in recent years as a powerful technique for structural studies of macromolecular complexes in their native cellular environment. However, the possibilities for recording tomographic tilt series in a high-throughput manner are limited, in part by the lamella-shaped samples. Here we utilize a geometrical sample model and optical image shift to record tens of tilt series in parallel, thereby saving time and gaining ...[more]

PMID: 36456783

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