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Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2

ABSTRACT:

SUBMITTER: Matthieu G Gagnon

PROVIDER: EMPIAR-11012 | biostudies-other |

REPOSITORIES: biostudies-other

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Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation.

Basu Ritwika S RS Sherman Michael B MB Gagnon Matthieu G MG

Nature communications 20220613 1

During translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-tRNAifMet) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit joining IF2 hydrolyzes GTP and, concomitant with inorganic phosphate (Pi) release, changes conformation facilitating fMet-tRNAifMet accommodation into the P site and transition of the 70 S ribosome initiation complex (70S-IC) to an elongation-competent ribosome ...[more]

PMID: 35697706

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