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Cryo-EM structure of human NKCC1 K289NA492EL671C bound with bumetanide

ABSTRACT:

SUBMITTER: Yongxiang Zhao

PROVIDER: EMPIAR-11049 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide.

Zhao Yongxiang Y Roy Kasturi K Vidossich Pietro P Cancedda Laura L De Vivo Marco M Forbush Biff B Cao Erhu E

Nature communications 20220518 1

Cation-chloride cotransporters (CCCs) NKCC1 and NKCC2 catalyze electroneutral symport of 1 Na+, 1 K+, and 2 Cl- across cell membranes. NKCC1 mediates trans-epithelial Cl- secretion and regulates excitability of some neurons and NKCC2 is critical to renal salt reabsorption. Both transporters are inhibited by the so-called loop diuretics including bumetanide, and these drugs are a mainstay for treating edema and hypertension. Here, our single-particle el ...[more]

PMID: 35585053

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