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Cryo-EM structure of the rigor state Jordan myosin-15-F-actin complex

ABSTRACT:

SUBMITTER: Gregory M. Alushin

PROVIDER: EMPIAR-11145 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.

Gong Rui R Jiang Fangfang F Moreland Zane G ZG Reynolds Matthew J MJ de Los Reyes Santiago Espinosa SE Gurel Pinar P Shams Arik A Heidings James B JB Bowl Michael R MR Bird Jonathan E JE Alushin Gregory M GM

Science advances 20220720 29

The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin regulatory machinery to stereocilia tips, myosin-15 directly nucleates actin filament ("F-actin") assembly, which is disrupted by a progressive hearing loss mutation (p.D1647G, "<i>jordan</i>"). Here, we present cryo-electron microscopy structures of myosin-15 bound to F-actin, providing a framework for ...[more]

PMID: 35857845

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