Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Thermostabilized human prestin in complex with chloride, sulfate or salicylate

ABSTRACT:

SUBMITTER: Tsukasa Kusakizako

PROVIDER: EMPIAR-11199 | biostudies-other |

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Publications

Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility.

Futamata Haon H Fukuda Masahiro M Umeda Rie R Yamashita Keitaro K Tomita Atsuhiro A Takahashi Satoe S Shikakura Takafumi T Hayashi Shigehiko S Kusakizako Tsukasa T Nishizawa Tomohiro T Homma Kazuaki K Nureki Osamu O

Nature communications 20221020 1

Outer hair cell elecromotility, driven by prestin, is essential for mammalian cochlear amplification. Here, we report the cryo-EM structures of thermostabilized prestin (Pres<sup>TS</sup>), complexed with chloride, sulfate, or salicylate at 3.52-3.63 Å resolutions. The central positively-charged cavity allows flexible binding of various anion species, which likely accounts for the known distinct modulations of nonlinear capacitance (NLC) by different anions. Comparisons of these Pres<sup>TS</sup ...[more]

PMID: 36266333

Similar Datasets

Human heparan sulfate polymerase complex EXT1-EXT2

Project description: Not available

| EMPIAR-11374 | biostudies-other

Cobalt Extraction from Sulfate/Chloride Media with Trioctyl(alkyl)phosphonium Chloride Ionic Liquids.

Project description:The application of phosphonium-based ionic liquids (ILs) on the selective extraction of cobalt is presented. The extraction mechanism is established, and different parameters of the process are evaluated. It has been found that it is possible to extract cobalt from aqueous solutions in sulfate media, with the addition of sodium chloride, using phosphonium ILs. The cobalt extraction was selective with respect to nickel and strongly dependent on the chloride concentration in the aqueous solution. The cobalt extraction is given by an anion exchange mechanism through an endothermic process. Cobalt extractions greater than 98% were obtained using the proposed methods. Cobalt stripping from the loaded IL phase using water was proved. Therefore, an alternative extraction process to traditional organic solvents is proposed. This alternative has additional advantages such as easy handling, lower costs in reagents and equipment, and risk reduction.

| S-EPMC7097900 | biostudies-literature

Structure of the human heparan sulfate polymerase complex EXT1-EXT2.

Project description:Heparan sulfates are complex polysaccharides that mediate the interaction with a broad range of protein ligands at the cell surface. A key step in heparan sulfate biosynthesis is catalyzed by the bi-functional glycosyltransferases EXT1 and EXT2, which generate the glycan backbone consisting of repeating N-acetylglucosamine and glucuronic acid units. The molecular mechanism of heparan sulfate chain polymerization remains, however, unknown. Here, we present the cryo-electron microscopy structure of human EXT1-EXT2, which reveals the formation of a tightly packed hetero-dimeric complex harboring four glycosyltransferase domains. A combination of in vitro and in cellulo mutational studies is used to dissect the functional role of the four catalytic sites. While EXT1 can catalyze both glycosyltransferase reactions, our results indicate that EXT2 might only have N-acetylglucosamine transferase activity. Our findings provide mechanistic insight into heparan sulfate chain elongation as a nonprocessive process and lay the foundation for future studies on EXT1-EXT2 function in health and disease.

| S-EPMC9675754 | biostudies-literature

Transport and Electrochemical Characteristics of CJMCED Homogeneous Cation Exchange Membranes in Sodium Chloride, Calcium Chloride, and Sodium Sulfate Solutions.

Project description:Recently developed and produced by Hefei Chemjoy Polymer Material Co. Ltd., homogeneous CJMC-3 and CJMC-5 cation-exchange membranes (CJMCED) are characterized. The membrane conductivity in NaCl, Na2SO4, and CaCl2 solutions, permeability in respect to the NaCl and CaCl2 diffusion, transport numbers, current-voltage curves (CVC), and the difference in the pH (DpH) of the NaCl solution at the desalination compartment output and input are examined for these membranes in comparison with a well-studied commercial Neosepta CMX cation-exchange membrane produced by Astom Corporation, Japan. It is found that the conductivity, CVC (at relatively low voltages), and water splitting rate (characterized by DpH) for both CJMCED membranes are rather close to these characteristics for the CMX membrane. However, the diffusion permeability of the CJMCED membranes is significantly higher than that of the CMX membrane. This is due to the essentially more porous structure of the CJMCED membranes; the latter reduces the counterion permselectivity of these membranes, while allowing much easier transport of large ions, such as anthocyanins present in natural dyes of fruit and berry juices. The new membranes are promising for use in electrodialysis demineralization of brackish water and natural food solutions.

| S-EPMC7463934 | biostudies-literature

Desulfonatronovibrio halophilus sp. nov., a novel moderately halophilic sulfate-reducing bacterium from hypersaline chloride-sulfate lakes in Central Asia.

Project description:Four strains of lithotrophic sulfate-reducing bacteria (SRB) have been enriched and isolated from anoxic sediments of hypersaline chloride-sulfate lakes in the Kulunda Steppe (Altai, Russia) at 2 M NaCl and pH 7.5. According to the 16S rRNA gene sequence analysis, the isolates were closely related to each other and belonged to the genus Desulfonatronovibrio, which, so far, included only obligately alkaliphilic members found exclusively in soda lakes. The isolates utilized formate, H(2) and pyruvate as electron donors and sulfate, sulfite and thiosulfate as electron acceptors. In contrast to the described species of the genus Desulfonatronovibrio, the salt lake isolates could only tolerate high pH (up to pH 9.4), while they grow optimally at a neutral pH. They belonged to the moderate halophiles growing between 0.2 and 2 M NaCl with an optimum at 0.5 M. On the basis of their distinct phenotype and phylogeny, the described halophilic SRB are proposed to form a novel species within the genus Desulfonatronovibrio, D. halophilus (type strain HTR1(T) = DSM24312(T) = UNIQEM U802(T)).

| S-EPMC3346931 | biostudies-literature

On the Effect of Sodium Chloride and Sodium Sulfate on Cold Denaturation.

Project description:Both sodium chloride and sodium sulfate are able to stabilize yeast frataxin, causing an overall increase of its thermodynamic stability curve, with a decrease in the cold denaturation temperature and an increase in the hot denaturation one. The influence of low concentrations of these two salts on yeast frataxin stability can be assessed by the application of a theoretical model based on scaled particle theory. First developed to figure out the mechanism underlying cold denaturation in water, this model is able to predict the stabilization of globular proteins provided by these two salts. The densities of the salt solutions and their temperature dependence play a fundamental role.

| S-EPMC4511003 | biostudies-literature

Sulfur speciation by HPLC-ICPQQQMS in complex human biological samples: taurine and sulfate in human serum and urine.

Project description:The advent of the triple quadrupole technology to the inductively coupled plasma mass spectrometry (ICPMS) technique has allowed a strong improvement in the accuracy and detection limits of ICPMS for non-metal elements such as sulfur by removing major polyatomic interferences. Up to now, there has been no report utilizing this development for sulfur speciation in complex human biological matrices. In the present report, we show the success of HPLC-ICPQQQMS for the simultaneous determination of two major sulfur metabolites, taurine and sulfate, in human urine and serum, by direct injection without the need for sample clean-up. The optimized chromatographic method was validated, tested for robustness, and applied for investigating the intra-individual variability in taurine urinary excretion in eight healthy volunteers over a period of 8 weeks. The limit of detection and limit of quantification for taurine determination was found to be 0.2 and 0.7 pmol, respectively. The concentrations found in the analyzed group of urine samples (n?=?64) had a range, mean, and SD of 0.6-99, 20.4, and 23.2 ?g mL-1 for taurine, and 115-1373, 616, and 259 ?g mL-1 for sulfate. Taurine was found to exhibit a much higher intra-individual variability than sulfate. The developed method can be applied in large-scale epidemiological studies and clinical studies in order to establish the potential cardioprotective effects of taurine. Graphical abstract ?.

| S-EPMC6132542 | biostudies-literature

Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia).

Project description:An antibiotic efflux gene cluster that confers resistance to chloramphenicol, trimethoprim, and ciprofloxacin has been identified in Burkholderia cenocepacia (genomovar III), an important cystic fibrosis pathogen. Five open reading frames have been identified in the cluster. There is apparently a single transcriptional unit, with llpE encoding a lipase-like protein, ceoA encoding a putative periplasmic linker protein, ceoB encoding a putative cytoplasmic membrane protein, and opcM encoding a previously described outer membrane protein. A putative LysR-type transcriptional regulatory gene, ceoR, is divergently transcribed upstream of the structural gene cluster. Experiments using radiolabeled chloramphenicol and salicylate demonstrated active efflux of both compounds in the presence of the gene cluster. Salicylate is an important siderophore produced by B. cepacia complex isolates, and both extrinsic salicylate and iron starvation appear to upregulate ceoR promoter activity, as does chloramphenicol. These results suggest that salicylate is a natural substrate for the efflux pump in B. cenocepacia and imply that the environment of low iron concentration in the cystic fibrosis lung can induce efflux-mediated resistance, even in the absence of antibiotic selective pressure.

| S-EPMC324543 | biostudies-literature

Structure of the human heparan sulfate polymerase complex EXT1-EXT2

Project description:Heparan sulfates are complex polysaccharides that mediate the interaction with a broad range of protein ligands at the cell surface. A key step in heparan sulfate biosynthesis is catalyzed by the bi-functional glycosyltransferases EXT1 and EXT2, which generate the glycan backbone consisting of repeating Nacetylglucosamine and glucuronic acid units. The molecular mechanism of heparan sulfate chain polymerization remains, however, unknown. Human EXT1 and EXT2, lacking the N-terminal transmembrane helix, were co-expressed as a secreted complex using human embryonic kidney cells (FreeStyle 293-F). We purified 0.5 mg of the complex from 900 mL suspension culture using two immobilized metal affinity chromatography steps and an interspersed protease treatment to cleave off the alkaline phosphatase fusion protein serving as a secretion signal. Mass spectrometry-based proteomic analysis confirmed the quality of the sample with EXT1 and EXT2 found to be the most abundant proteins, and being present in comparable amounts.

2022-12-09 | PXD034871 | Pride

Cyclo-benzaprinium salicylate.

Project description:In the title mol-ecular salt [systematic name: 3-(5H-di-benzo[a,d]cyclo-hepten-5-yl-idene)-N,N-dimethyl-1-propanaminium 2-hy-droxy-benzoate], C(20)H(22)N(+)·C(7)H(5)O(3) (-), the benzene rings of the cyclo-benzaprinium cation are inclined with a dihedral angle of 61.66 (7)°. An intra-molecular O-H⋯O hydrogen bond occurs within the salicylate anion, generating an S(6) ring. In the crystal, the cation and anion are linked by an N-H⋯O inter-action.

| S-EPMC3152117 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data