A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed-refractory multiple myeloma
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ABSTRACT:
SUBMITTER: Alwin Krämer
PROVIDER: EMPIAR-11243 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed/refractory multiple myeloma.
Dittrich Tobias T Köhrer Sebastian S Schorb Martin M Haberbosch Isabella I Börmel Mandy M Goldschmidt Hartmut H Pajor Gabor G Müller-Tidow Carsten C Raab Marc S MS Hegenbart Ute U Schönland Stefan O SO Schwab Yannick Y Krämer Alwin A
Cell reports methods 20221101 11
Electron microscopy is the gold standard to characterize centrosomal ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. We therefore developed a generalizable, semi-automated high-throughput electron tomography strategy to study centrosome aberrations in sparse patient-derived cancer cells at nanoscale. As proof of principle, we present electron tomography data on 455 centrioles of CD138<sup>pos</sup> plasma cells from one patient with r ...[more]
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