Dataset Information
Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GTP
Ontology highlight
ABSTRACT:
SUBMITTER: Denis Chrétien
PROVIDER: EMPIAR-11253 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts.
eLife 20221212
Microtubules are tubes of about 25 nm in diameter that are critically involved in a variety of cellular functions, including motility, compartmentalization, and division. They are considered as pseudo-helical polymers whose constituent αβ-tubulin heterodimers share lateral homotypic interactions, except at one unique region called the seam. Here, we used a segmented sub-tomogram averaging strategy to reassess this paradigm and analyze the organization of the αβ-tubulin heterodimers in microtubul ...[more]