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Cryo-electron tomography of microtubules assembled in Xenopus egg cytoplasmic extracts

ABSTRACT:

SUBMITTER: Denis Chrétien

PROVIDER: EMPIAR-11263 | biostudies-other |

REPOSITORIES: biostudies-other

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Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts.

Guyomar Charlotte C Bousquet Clément C Ku Siou S Heumann John M JM Guilloux Gabriel G Gaillard Natacha N Heichette Claire C Duchesne Laurence L Steinmetz Michel O MO Gibeaux Romain R Chrétien Denis D

eLife 20221212

Microtubules are tubes of about 25 nm in diameter that are critically involved in a variety of cellular functions, including motility, compartmentalization, and division. They are considered as pseudo-helical polymers whose constituent αβ-tubulin heterodimers share lateral homotypic interactions, except at one unique region called the seam. Here, we used a segmented sub-tomogram averaging strategy to reassess this paradigm and analyze the organization of the αβ-tubulin heterodimers in microtubul ...[more]

PMID: 36503602

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