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Cryo-electron tomography of microtubules assembled in Xenopus egg cytoplasmic extracts


ABSTRACT:

SUBMITTER: Denis Chrétien 

PROVIDER: EMPIAR-11263 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in <i>Xenopus</i> egg cytoplasmic extracts.

Guyomar Charlotte C   Bousquet Clément C   Ku Siou S   Heumann John M JM   Guilloux Gabriel G   Gaillard Natacha N   Heichette Claire C   Duchesne Laurence L   Steinmetz Michel O MO   Gibeaux Romain R   Chrétien Denis D  

eLife 20221212


Microtubules are tubes of about 25 nm in diameter that are critically involved in a variety of cellular functions, including motility, compartmentalization, and division. They are considered as pseudo-helical polymers whose constituent αβ-tubulin heterodimers share lateral homotypic interactions, except at one unique region called the seam. Here, we used a segmented sub-tomogram averaging strategy to reassess this paradigm and analyze the organization of the αβ-tubulin heterodimers in microtubul  ...[more]

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