Topological Crossing in the Misfolded Tetrahymena Ribozyme Resolved by Cryo-EM
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ABSTRACT:
SUBMITTER: Kaiming Zhang
PROVIDER: EMPIAR-11267 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Topological crossing in the misfolded <i>Tetrahymena</i> ribozyme resolved by cryo-EM.
Li Shanshan S Palo Michael Z MZ Pintilie Grigore G Zhang Xiaojing X Su Zhaoming Z Kappel Kalli K Chiu Wah W Zhang Kaiming K Das Rhiju R
Proceedings of the National Academy of Sciences of the United States of America 20220906 37
The <i>Tetrahymena</i> group I intron has been a key system in the understanding of RNA folding and misfolding. The molecule folds into a long-lived misfolded intermediate (M) <i>in vitro</i>, which has been known to form extensive native-like secondary and tertiary structures but is separated by an unknown kinetic barrier from the native state (N). Here, we used cryogenic electron microscopy (cryo-EM) to resolve misfolded structures of the <i>Tetrahymena</i> L-21 ScaI ribozyme. Maps of three M ...[more]
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