Dataset Information
Initiation complex consisting of E. coli 70S ribosome with AAm6A mRNA codon in the A-site incubated with a ternary complex containing cognate acylated tRNA(Lys)
Ontology highlight
ABSTRACT:
SUBMITTER: Lukasz Koziej
PROVIDER: EMPIAR-11288 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Modulation of translational decoding by m<sup>6</sup>A modification of mRNA.
Nature communications 20230808 1
N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. m<sup>6</sup>A in the mRNA coding regions inhibits translation elongation. Here, we show how m<sup>6</sup>A modulates decoding in the bacterial translation system using a combination of rapid kinetics, smFRET and single-particle cryo-EM. We show that, while the modification does not impair the initial binding of aminoacyl-tRNA to the ribosome, in the pres ...[more]