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Cryo pFIB/SEM of PEG beads (test sample)

ABSTRACT:

SUBMITTER: Maud Dumoux

PROVIDER: EMPIAR-11414 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-plasma FIB/SEM volume imaging of biological specimens.

Dumoux Maud M Glen Thomas T Smith Jake L R JLR Ho Elaine M L EML Perdigão Luis M A LMA Pennington Avery A Klumpe Sven S Yee Neville B Y NBY Farmer David Andrew DA Lai Pui Y A PYA Bowles William W Kelley Ron R Plitzko Jürgen M JM Wu Liang L Basham Mark M Clare Daniel K DK Siebert C Alistair CA Darrow Michele C MC Naismith James H JH Grange Michael M

eLife 20230221

Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in cells and tissues while maintaining constituents' hydration shells for in situ structural biology downstream. However, the application of serial FIB/SEM imaging of non-stained cryogenic biological samples is limited due to low contrast, curtaini ...[more]

PMID: 36805107

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Project description: Not available

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