Ontology highlight
ABSTRACT:
SUBMITTER: Maud Dumoux
PROVIDER: EMPIAR-11414 | biostudies-other |
REPOSITORIES: biostudies-other
Dumoux Maud M Glen Thomas T Smith Jake L R JLR Ho Elaine M L EML Perdigão Luis M A LMA Pennington Avery A Klumpe Sven S Yee Neville B Y NBY Farmer David Andrew DA Lai Pui Y A PYA Bowles William W Kelley Ron R Plitzko Jürgen M JM Wu Liang L Basham Mark M Clare Daniel K DK Siebert C Alistair CA Darrow Michele C MC Naismith James H JH Grange Michael M
eLife 20230221
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in cells and tissues while maintaining constituents' hydration shells for in situ structural biology downstream. However, the application of serial FIB/SEM imaging of non-stained cryogenic biological samples is limited due to low contrast, curtaini ...[more]