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Cryo serial FIB/SEM of mouse heart tissue

ABSTRACT:

SUBMITTER: Maud Dumoux 

PROVIDER: EMPIAR-11420 | biostudies-other |

REPOSITORIES: biostudies-other

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Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structures in cells and tissues while maintaining constituents' hydration shells for in situ structural biology downstream. However, the application of serial FIB/SEM imaging of non-stained cryogenic biological samples is limited due to low contrast, curtaini  ...[more]

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