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Cryo-EM structure of pre-consensus elemental paused elongation complex

ABSTRACT:

SUBMITTER: Seth A Darst

PROVIDER: EMPIAR-11506 | biostudies-other |

REPOSITORIES: biostudies-other

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An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.

Kang Jin Young JY Mishanina Tatiana V TV Bao Yu Y Chen James J Llewellyn Eliza E Liu James J Darst Seth A SA Landick Robert R

Proceedings of the National Academy of Sciences of the United States of America 20230216 8

Transcriptional pausing underpins the regulation of cellular RNA synthesis, but its mechanism remains incompletely understood. Sequence-specific interactions of DNA and RNA with the dynamic, multidomain RNA polymerase (RNAP) trigger reversible conformational changes at pause sites that temporarily interrupt the nucleotide addition cycle. These interactions initially rearrange the elongation complex (EC) into an elemental paused EC (ePEC). ePECs can form longer-lived PECs by further rearrangement ...[more]

PMID: 36795753

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