Dataset Information
Archaeal DNA ligase and heterotrimeric PCNA with ligatable nicked DNA
Ontology highlight
ABSTRACT:
SUBMITTER: Aleksandr Sverzhinsky
PROVIDER: EMPIAR-11552 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structures and biochemical insights into heterotrimeric PCNA regulation of DNA ligase.
Structure (London, England : 1993) 20211126 3
DNA ligases act in the final step of many DNA repair pathways and are commonly regulated by the DNA sliding clamp proliferating cell nuclear antigen (PCNA), but there are limited insights into the physical basis for this regulation. Here, we use single-particle cryoelectron microscopy (cryo-EM) to analyze an archaeal DNA ligase and heterotrimeric PCNA in complex with a single-strand DNA break. The cryo-EM structures highlight a continuous DNA-binding surface formed between DNA ligase and PCNA th ...[more]