Cryo-EM structure of human TFIIIC bound and unbound to tRNA gene
Ontology highlight
ABSTRACT:
SUBMITTER: Christoph W Müller
PROVIDER: EMPIAR-11762 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural insights into human TFIIIC promoter recognition.
Seifert-Davila Wolfram W Girbig Mathias M Hauptmann Luis L Hoffmann Thomas T Eustermann Sebastian S Müller Christoph W CW
Science advances 20230707 27
Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The τB module recognizes the B-box via DNA shape and sequence readout through the assembl ...[more]
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