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Sulfolobus acidocaldarius s-layer SlaA

ABSTRACT:

SUBMITTER: Bertram Daum

PROVIDER: EMPIAR-11788 | biostudies-other |

REPOSITORIES: biostudies-other

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Structure of the two-component S-layer of the archaeon <i>Sulfolobus acidocaldarius</i>.

Gambelli Lavinia L McLaren Mathew M Conners Rebecca R Sanders Kelly K Gaines Matthew C MC Clark Lewis L Gold Vicki A M VAM Kattnig Daniel D Sikora Mateusz M Hanus Cyril C Isupov Michail N MN Daum Bertram B

eLife 20240122

Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo e ...[more]

PMID: 38251732

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Sulfolobus acidocaldarius s-layer SlaA cryoET dataset

Project description: Not available

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Structure of the two-component S-layer of the archaeon <i>Sulfolobus acidocaldarius</i>.

Project description:Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of Sulfolobus acidocaldarius. The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly.

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