RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease
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ABSTRACT:
SUBMITTER: Hiroshi Nishimasu
PROVIDER: EMPIAR-11824 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease.
Kato Kazuki K Okazaki Sae S Schmitt-Ulms Cian C Jiang Kaiyi K Zhou Wenyuan W Ishikawa Junichiro J Isayama Yukari Y Adachi Shungo S Nishizawa Tomohiro T Makarova Kira S KS Koonin Eugene V EV Abudayyeh Omar O OO Gootenberg Jonathan S JS Nishimasu Hiroshi H
Science (New York, N.Y.) 20221103 6622
The type III-E CRISPR-Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo-electron microscopy structures of the Cas7-11-crRNA-Csx29 complex with and without target RNA (tgRNA), and demonstrate that tgRNA binding induces conformational changes in Csx29. Biochemical experiments revealed tgRNA-dependent cleavage of the accessory protein Csx30 by Csx29. Reconstitution of the system in bacteria showed that Csx30 cleavage ...[more]
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