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Cryo-EM of HCoV-NL63 5' proximal stem-loop 5

ABSTRACT:

SUBMITTER: Rhiju Das

PROVIDER: EMPIAR-11848 | biostudies-other |

REPOSITORIES: biostudies-other

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Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses.

Kretsch Rachael C RC Xu Lily L Zheludev Ivan N IN Zhou Xueting X Huang Rui R Nye Grace G Li Shanshan S Zhang Kaiming K Chiu Wah W Das Rhiju R

Proceedings of the National Academy of Sciences of the United States of America 20240301 10

Coronavirus genomes sequester their start codons within stem-loop 5 (SL5), a structured, 5' genomic RNA element. In most alpha- and betacoronaviruses, the secondary structure of SL5 is predicted to contain a four-way junction of helical stems, some of which are capped with UUYYGU hexaloops. Here, using cryogenic electron microscopy (cryo-EM) and computational modeling with biochemically determined secondary structures, we present three-dimensional structures of SL5 from six coronaviruses. The SL ...[more]

PMID: 38427602

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