Ontology highlight
ABSTRACT:
SUBMITTER: Georgios Skiniotis
PROVIDER: EMPIAR-11856 | biostudies-other |
REPOSITORIES: biostudies-other
Papasergi-Scott Makaía M MM Pérez-Hernández Guillermo G Batebi Hossein H Gao Yang Y Eskici Gözde G Seven Alpay B AB Panova Ouliana O Hilger Daniel D Casiraghi Marina M He Feng F Maul Luis L Gmeiner Peter P Kobilka Brian K BK Hildebrand Peter W PW Skiniotis Georgios G
Nature 20240313 8014
G-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by stimulating guanine nucleotide exchange in the Gα subunit<sup>1</sup>. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines the progression of ensembles of pre-steady-state intermediates of a GPCR-G-protein complex. By monitoring the transitions of the stimulatory G<sub>s</sub> protein in complex with the β<sub>2</sub>-adrenergic receptor at short sequential time points after GTP additio ...[more]