Unveiling the ultrastructural landscape of extracellular matrix via lift-out cryo-FIBSEM and cryo-ET
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ABSTRACT:
SUBMITTER: Florian K.M. Schur
PROVIDER: EMPIAR-11897 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix.
Zens Bettina B Fäßler Florian F Hansen Jesse M JM Hauschild Robert R Datler Julia J Hodirnau Victor-Valentin VV Zheden Vanessa V Alanko Jonna J Sixt Michael M Schur Florian K M FKM
The Journal of cell biology 20240320 6
The extracellular matrix (ECM) serves as a scaffold for cells and plays an essential role in regulating numerous cellular processes, including cell migration and proliferation. Due to limitations in specimen preparation for conventional room-temperature electron microscopy, we lack structural knowledge on how ECM components are secreted, remodeled, and interact with surrounding cells. We have developed a 3D-ECM platform compatible with sample thinning by cryo-focused ion beam milling, the lift-o ...[more]
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