Dataset Information
Cryo-ET tilt series from mouse islets lift-out sample
Ontology highlight
ABSTRACT:
SUBMITTER: Yichun Wu
PROVIDER: EMPIAR-11469 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
A practical multicellular sample preparation pipeline broadens the application of in situ cryo-electron tomography.
Journal of structural biology 20230516 3
The structural studies of macromolecules in their physiological context, particularly in tissue, is constrained by the bottleneck of sample preparation. In this study, we present a practical pipeline for preparing multicellular samples for cryo-electron tomography. The pipeline comprises sample isolation, vitrification, and lift-out-based lamella preparation using commercially available instruments. We demonstrate the efficacy of our pipeline by visualizing pancreatic β cells from mouse islets a ...[more]