Dataset Information
Cryo electron tomography of human Alzheimer's disease brain tissue
Ontology highlight
ABSTRACT:
SUBMITTER: Yi-Wei Chang
PROVIDER: EMPIAR-11920 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling.
Nature communications 20240326 1
Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following fixation, staining, and sectioning, which limit resolution and introduce artifacts. Alternatively, cryo-electron tomography (cryo-ET) allows higher resolution imaging of unfixed cellular samples while preserving architecture, but it requires samples to be vitreous and thin enough for transmission EM. Due to these requirements, cryo-ET has yet to be employed to investigate unfixed, never ...[more]