Project description:Single-stranded DNA bacteriophages of the Microviridae family are emerging as major components of the global virosphere, found abundantly across diverse habitats. We have identified and thoroughly characterized a new type of microvirus, Ebor, which infects the freshwater mixotrophic model bacterium Rhodobacter capsulatus. Here, we present raw LC-MS/MS data of partially purified virions of Ebor propagated on R. capsulatus strain SB1003. The sample E025 corresponds to ion-exchange purified virions, the sample D972 TOP to upper band obtained by CsCl gradient ultracentrifugation containing mostly flagella and the sample D972 BOTTOM to lower band of the respective centrifugation containing native virions.

2024-05-06 | MSV000094685 | MassIVE

Microvirus

Project description:viral metagenome of piglets

| PRJNA1057724 | ENA

The Antimicrobial Activity of a Carbon Monoxide Releasing Molecule (EBOR-CORM-1) Is Shaped by Intraspecific Variation within <i>Pseudomonas aeruginosa</i> Populations.

Project description:Carbon monoxide releasing molecules (CORMs) have been suggested as a new synthetic class of antimicrobials to treat bacterial infections. Here we utilized a novel EBOR-CORM-1 ([NEt4][MnBr2(CO)4]) capable of water-triggered CO-release, and tested its efficacy against a collection of clinical Pseudomonas aeruginosa strains that differ in infection-related virulence traits. We found that while EBOR-CORM-1 was effective in clearing planktonic and biofilm cells of P. aeruginosa strain PAO1 in a concentration dependent manner, this effect was less clear and varied considerably between different P. aeruginosa cystic fibrosis (CF) lung isolates. While a reduction in cell growth was observed after 8 h of CORM application, either no effect or even a slight increase in cell densities and the amount of biofilm was observed after 24 h. This variation could be partly explained by differences in bacterial virulence traits: while CF isolates showed attenuated in vivo virulence and growth compared to strain PAO1, they formed much more biofilm, which could have potentially protected them from the CORM. Even though no clear therapeutic benefits against a subset of isolates was observed in an in vivo wax moth acute infection model, EBOR-CORM-1 was more efficient at reducing the growth of CF isolate co-culture populations harboring intraspecific variation, in comparison with efficacy against more uniform single isolate culture populations. Together these results suggest that CORMs could be effective at controlling genetically diverse P. aeruginosa populations typical for natural chronic CF infections and that the potential benefits of some antibiotics might not be observed if tested only against clonal bacterial populations.

| S-EPMC5809400 | biostudies-literature

Trapping intermediate MLCT states in low-symmetry {Ru(bpy)} complexes.

Project description:The picosecond excited state dynamics of [Ru(tpm)(bpy)(NCS)]+ (RubNCS+ ) and [Ru(tpm)(bpy)(CN)]+ (RubCN+ ) (tpm = tris(1-pyrazolyl)methane, bpy = 2,2'-bipyridine) have been analyzed by means of transient absorption measurements and spectroelectrochemistry. Emissive 3MLCTs with (GS)HOMO(h+)-(GS)LUMO(e-) configurations are the lowest triplet excited states regardless of whether 387 or 505 nm photoexcitation is used. 387 nm photoexcitation yields, after a few picoseconds, the emissive 3MLCTs. In contrast, 505 nm photoexcitation populates an intermediate excited state that we assign as a 3MLCT state, in which the hole sits in a metal-centered orbital of different symmetry, prior to its conversion to the emissive 3MLCTs. The disparities in terms of electronic configuration between the intermediate and the emissive 3MLCTs have two important consequences. On one hand, both states feature very different fingerprint absorptions in transient absorption measurements. On the other hand, the reconfiguration is impeded by a kinetic barrier. As such, the conversion is followed spectroscopically and kinetically on the 300 ps timescale.

| S-EPMC5674176 | biostudies-literature

Complete Genome Sequences of Two Rhodobacter Strains.

Project description:We report the complete genome sequences of two strains of the Alphaproteobacteria genus Rhodobacter, Rhodobacter blasticus 28/5, the source of the commercially available enzyme RsaI, and a new isolate of Rhodobacter sphaeroides 2.4.1. Both strains contain multiple restriction-modification systems, and their DNA methylation motifs are included in this report.

| S-EPMC6256690 | biostudies-literature

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