Unknown

Dataset Information

0

MiR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner


ABSTRACT: The circular RNA (circRNA) Cdr1as is conserved across mammals and highly expressed in neurons, where it directly interacts with microRNA miR-7. However, the biological function of this interaction is unknown. Here, using primary cortical murine neurons, we demonstrate that stimulating neurons by sustained depolarization rapidly induces two-fold transcriptional up-regulation of Cdr1as and strong post-transcriptional stabilization of miR-7. Cdr1as loss causes doubling of glutamate release from stimulated synapses and increased frequency and duration of local neuronal bursts. Moreover, periodicity of neuronal networks increases, and synchronicity is impaired. Strikingly, these effects are reverted by sustained expression of miR-7, which also clears Cdr1as molecules from neuronal projections. Consistently, without Cdr1as, transcriptomic changes caused by miR-7 overexpression are stronger (including miR-7-targets down-regulation) and enriched in secretion/synaptic plasticity pathways. Altogether, our results suggest that in cortical neurons Cdr1as buffers miR-7 activity to control glutamatergic excitatory transmission and neuronal connectivity important for long-lasting synaptic adaptations.

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:  

PROVIDER: S-BSST1417 | biostudies-other |

REPOSITORIES: biostudies-other

Similar Datasets

2024-04-26 | GSE224184 | GEO
| PRJNA930025 | ENA
| S-EPMC6544175 | biostudies-literature
| S-EPMC5758559 | biostudies-literature
2024-01-12 | GSE189353 | GEO
| S-EPMC8027626 | biostudies-literature
2024-01-12 | GSE189348 | GEO
2024-01-12 | GSE189319 | GEO
2024-01-12 | GSE189313 | GEO
| S-EPMC5826268 | biostudies-literature