Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner

ABSTRACT: To investigate interactions of Cdr1as and miR-7 in mouse neuron, we cultured primary mouse cell in which each target gene has been knocked out from C57BL/6N mouse and overexpression of pri-miR-7a

ORGANISM(S): Mus musculus

PROVIDER: GSE224184 | GEO | 2024/04/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner

Project description:miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner

| PRJNA930025 | ENA

miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner

Project description: Not available

| S-BSST1417 | biostudies-other

miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner.

Project description:The circular RNA (circRNA) Cdr1as is conserved across mammals and highly expressed in neurons, where it directly interacts with microRNA miR-7. However, the biological function of this interaction is unknown. Here, using primary cortical murine neurons, we demonstrate that stimulating neurons by sustained depolarization rapidly induces two-fold transcriptional upregulation of Cdr1as and strong post-transcriptional stabilization of miR-7. Cdr1as loss causes doubling of glutamate release from stimulated synapses and increased frequency and duration of local neuronal bursts. Moreover, the periodicity of neuronal networks increases, and synchronicity is impaired. Strikingly, these effects are reverted by sustained expression of miR-7, which also clears Cdr1as molecules from neuronal projections. Consistently, without Cdr1as, transcriptomic changes caused by miR-7 overexpression are stronger (including miR-7-targets downregulation) and enriched in secretion/synaptic plasticity pathways. Altogether, our results suggest that in cortical neurons Cdr1as buffers miR-7 activity to control glutamatergic excitatory transmission and neuronal connectivity important for long-lasting synaptic adaptations.

| S-EPMC11239925 | biostudies-literature

Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis [RIP-Seq]

Project description:Metastasis is the primary cause of death of cancer patients. Dissecting mechanisms governing metastatic spread may uncover important tumor biology and/or yield promising therapeutic insights. Here we investigated the role of circular RNAs (circRNA) in metastasis, using melanoma as a model aggressive tumor. We identified silencing of Cerebellar Degeneration Related 1 (CDR1as), a regulator of the microRNA miR-7, as a hallmark of melanoma progression. We find that CDR1as depletion results from epigenetic silencing of its originating lincRNA, and promotes invasion in vitro and metastasis in vivo, through a miR-7-independent, IGF2BP3-mediated mechanism. Moreover, CDR1as levels identify cellular states associated with distinct therapeutic responses. Our study reveals functional, prognostic and predictive roles for CDR1as and expose circRNAs as key players in metastasis.

2020-01-13 | GSE138710 | GEO

Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis [RNA-Seq]

2020-01-13 | GSE138412 | GEO

Comprehensive transcriptome analysis regarding CDR1AS expression

Project description:We determined the gene expression changes by CDR1AS expression in colon cancer cells

2020-02-01 | GSE125687 | GEO

Comprehensive transcriptome analysis regarding CDR1AS expression

Project description:Comprehensive transcriptome analysis regarding CDR1AS expression

| PRJNA599175 | ENA

Comprehensive transcriptome analysis regarding CDR1AS expression

Project description:Comprehensive transcriptome analysis regarding CDR1AS expression

| PRJNA517166 | ENA

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function [miRNA-seq]

Project description:Hundreds of circular RNAs (circRNAs) are highly abundant in the mammalian brain, often with conserved expression. Here we show that the circRNA Cdr1as is massively bound by the microRNAs (miRNAs) miR-7 and miR-671 in human and mouse brains. When the Cdr1as locus was removed from the mouse genome, knockout animals displayed impaired sensorimotor gating-a deficit in the ability to filter out unnecessary information-which is associated with neuropsychiatric disorders. Electrophysiological recordings revealed dysfunctional synaptic transmission. Expression of miR-7 and miR-671 was specifically and posttranscriptionally misregulated in all brain regions analyzed. Expression of immediate early genes such as Fos, a direct miR-7 target, was enhanced in Cdr1as-deficient brains, providing a possible molecular link to the behavioral phenotype. Our data indicate an in vivo loss-of-function circRNA phenotype and suggest that interactions between Cdr1as and miRNAs are important for normal brain function.

2017-08-11 | GSE93129 | GEO

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function [rRNA-depleted RNA-Seq]

2017-08-11 | GSE100265 | GEO