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CRISPR screening of porcine sgRNA library identified host factors facilitating Japanese encephalitis virus replication

ABSTRACT: We design a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO v1.0) library containing 85,674 single guide RNAs targeting 17,743 protein-coding genes, 11,053 long ncRNAs, and 551 microRNAs. Subsequently, we use the PigGeCKO library to identify key host factors facilitating Japanese encephalitis virus infection in porcine cells.

ORGANISM(S): Sus scrofa (pig)

SUBMITTER: Shengsong Xie 

PROVIDER: S-BSST510 | biostudies-other |

REPOSITORIES: biostudies-other

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