Simulated structurally variable Nuclear Pore Complexes for Microscopy simulations

ABSTRACT: The Nuclear Pore Complex (NPC) is a massive, 8-fold symmetrical protein assembly that enables macromolecular exchange between the cytoplasm and nucleus. The average architecture of proteins within the NPC has been resolved with pseudo-atomic precision, however observed NPC heterogeneities such as varying diameters, elongated shapes, 9-fold symmetry, and irregular shapes evidence a high degree of divergence from this average. Single Molecule Localization Microscopy (SMLM) images NPCs at protein-level resolution, whereupon image analysis software studies their heterogeneity. Antithetically, NPCs have been used as a reference standard for SMLM due to their abundance, size, and symmetry. Ground truth annotations would ultimately aid both lines of research, however SMLM noise impedes such annot

ORGANISM(S): Homo sapiens (human)

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PROVIDER: S-BSST842 | biostudies-other |

REPOSITORIES: biostudies-other