Data integration from two microarray platforms identifies genetic inactivation of RIC8A in a breast cancer cell line

ABSTRACT: Using array comparative genomic hybridization (aCGH), a large number of deleted genomic regions have been identified in human cancers. However, subsequent efforts to identify target genes selected for inactivation in these regions have often been challenging. We integrated here genome-wide copy number data with gene expression data and non-sense mediated mRNA decay rates in breast cancer cell lines to prioritize gene candidates that are likely to be tumour suppressor genes inactivated by bi-allelic genetic events. The candidates were sequenced to identify potential mutations. This integrated genomic approach led to the identification of RIC8A at 11p15 as a putative candidate target gene for the genomic deletion in the ZR-75-1 breast cancer cell line. We identified a truncating mutation in

ORGANISM(S): Homo sapiens

SUBMITTER: Muggerud AA 

PROVIDER: S-ECPF-GEOD-15477 | biostudies-other | 2009

REPOSITORIES: biostudies-other