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Gene expression profiles elicited by estradiol and endoxifen in MCF7 parental and ER-beta expressing breast cancer cells

ABSTRACT: We have previously demonstrated that endoxifen is the most important tamoxifen metabolite responsible for eliciting the anti-estrogenic effects of this drug in breast cancer cells expressing estrogen receptor-alpha. However, the relevance of estrogen receptor-beta in mediating endoxifen action has yet to be explored. Therefore, the goals of this study were to determine the differences in the global gene expression profiles elicited by estradiol treatment and endoxifen between parental MCF7 breast cancer cells (expressing estrogen receptor alpha only) and MCF7 cells stably expressing estrogen receptor beta. Total RNA was isolated from parental or estrogen-receptor beta expressing MCF7 cells following 24 hour treatments with either ethanol vehicle, 1nM 17-beta-estradiol or 1nM estradiol plus 40nM endoxifen. All studies were conducted in biological replicates of 2.

ORGANISM(S): Homo sapiens

SUBMITTER: Wu X

PROVIDER: S-ECPF-GEOD-27375 | biostudies-other | 2011

REPOSITORIES: biostudies-other

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Gene expression profiles elicited by estradiol and endoxifen in MCF7 parental and ER-beta expressing breast cancer cells

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MCF7 breast cancer cell response to estradiol

Project description:The experiment was designed to uncover the response to estradiol (E2) in MCF7 breast cancer cells. The first step was to create a reference sample in a ligand free environment. For that, the cells were placed into estradiol free media for 3 days, which reduced the binding between ER-alpha and E2. The cells were then ready to be re-exposed to E2. Following the introduction of E2, the resultant changes were tracked by multiple ChIP-seq experiments. The experiments were performed at 0, 5, 10, 20, 40, 80, 160, 320, 640 and 1280 minutes after the stimulation. Each ChIP-seq experiment was carried out with a different antibody to measure genome-wide changes in genomic occupancy of their specific protein targets. Specifically, the studied protein factors and histone modifications were: ER-alpha, H3K4me3, and H2AZ. Other previously published data from the same set of experiments are available for Pol-II ChIP-Seq and RNA-Seq (GEO accession GSE62789 and GSE44800).

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